500mL of tap water
3g Marmite
5g dark brown sugar
250mg Penicillin
250mg Cephalexin

Microwave everything but the antibiotics separately in order to sterilize. Once the liquid culture is cool, add the antibiotics dissolved in sterilized water. Divide the liquid culture and add the innoculum.
Pleurotus ostreatus and Pleurotus djamor were the two species used in this video, but this method works great for any filamentous fungi!


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